The aim of the investigation was to explore the characterization of peptide fragment of neurosecretory protein VGF precursor (373-417) in the brain. We made the antibodies against the peptide and stained the mouse and rat brain tissue. We found the antibodies recognized nuclear protein in the neural cells in cerebral cortex, alveus hippocamp, nigra by immunohistochemistry and western blot. These results suggested the VGF peptide might act as nuclear protein.