Papers

Basic information

Name OZAWA Hitoshi

Title

Transgenic expression of enhanced green fluorescent protein enables direct visualization for physiological studies of vasopressin neurons and isolated nerve terminals of the rat

Author

Yoichi Ueta,Hiroaki Fujihara,Ryota Serino,Govindan Dayanithi,Hitoshi Ozawa,Ken-Ichi Matsuda,Mitsuhiro Kawata,Junko Yamada,Shinya Ueno,Atsuo Fukuda,David Murphy

Sole or Joint Author

 

Journal

Endocrinology

Publisher

 

All Volumes

 

All Pages

 

Volume

146

Number

1

Starting Page

406

Ending Page

413

Publication Date

2005-01

Referee Paper

Refereed

Invited Paper

Not invited

Language

English

MISC Class

 

Publishing Type

Research paper (scientific journal)

ISSN

 

ID:DOI

10.1210/en.2004-0830

ID:NAID

 

ID:PMID

 

URL

Description

We have generated transgenic rats expressing an arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene. The expression of the eGFP gene and strong fluorescence were observed in the supraoptic nucleus (SON), the paraventricular nucleus (PVN), and the suprachiasmatic nucleus (SCN) in transgenic rats. The hypothalamoneurohypophyseal tract, isolated SON neurons, and isolated axon terminals in the neurohypophysis also showed robust eGFP fluorescence. Water deprivation for 2 d increased the fluorescence of the eGFP in the SON and the PVN but not the SCN. The whole-cell patch-clamp technique was then used to record the electrical activities specifically identifying eGFP-expressing SON, PVN, and SCN AVP neurons in in vitro brain slice preparations. The AVP-eGFP transgenic rats are a unique new tool with which to study the physiological role of AVP-secreting neurons in the central nervous system and the dynamics of the regulation of AVP secretion in the living neurons and their axon terminals.

ID:JGlobalID

 

arXiv ID

 

Put Code of ORCID

 

DBLP ID

 

WekoID of OpenDepo