Papers

Basic information

Name OZAWA Hitoshi

Title

Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres

Author

Masateru Nagae,Takumi Ikeda,Yasuo Mikami,Hitoshi Hase,Hitoshi Ozawa,Ken-Ichi Matsuda,Hirotaka Sakamoto,Yasuhiko Tabata,Mitsuhiro Kawata,Toshikazu Kubo

Sole or Joint Author

 

Journal

Tissue Engineering

Publisher

 

All Volumes

 

All Pages

 

Volume

13

Number

1

Starting Page

147

Ending Page

158

Publication Date

2007-01

Referee Paper

Refereed

Invited Paper

Not invited

Language

English

MISC Class

 

Publishing Type

Research paper (international conference proceedings)

ISSN

 

ID:DOI

10.1089/ten.2006.0042

ID:NAID

 

ID:PMID

 

URL

Description

This study evaluated the regenerative effects of platelet-rich plasma (PRP) for the degenerated intervertebral disc (IVD) in vivo. After induction of IVD degeneration in rabbits, we prepared PRP by centrifuging blood obtained from these rabbits. These PRP were injected into the nucleus pulposus (NP) of the degenerated IVDs after impregnation into gelatin hydrogel microspheres that can immobilize PRP growth factors physiochemically and release them in a sustained manner with the degradation of the microspheres. As controls, microspheres impregnated with phosphate-buffered saline (PBS) and PRP without microspheres were similarly injected. Histologically, notable progress in IVD degeneration with time courses was observed in the PBS control, PRP-only, and sham groups. In contrast, progress was remarkably suppressed over the 8-week period in the PRP group. Moreover, in immunohistochemistry, intense immunostaining for proteoglycan in the NP and inner layer of the annulus fibrosus was observed 8 weeks after administration of PRP-impregnated microspheres. Almost all microspheres were indistinct 8 weeks after the injection, and there were no apparent side effects in this study. Our results suggest that the combined administration of PRP and gelatin hydrogel microspheres into the IVD may be a promising therapeutic modality for IVD degeneration. © Mary Ann Liebert, Inc.

ID:JGlobalID

 

arXiv ID

 

Put Code of ORCID

 

DBLP ID

 

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